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Rev Clin Periodoncia Implantol Rehabil Oral. 2008;1:53-56
TRABAJO DE INVESTIGACIÓN [PDF]

Caracterización de Perfiles Proteicos y Peptídicos en Periodontitis Crónica

Characterization of Protein and Peptide Profiles in Chronic Periodontitis

Hernández M1,3, Obregón F1, Pozo P1, Barriga A2, Valenzuela MA2.

1Laboratorio de Biología Periodontal, Departamento de Odontología Conservadora, Facultad de Odontología, Universidad de Chile. Chile.
2Depto. de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile. Chile.
3Departamento de Patología, Facultad de Odontología, Universidad de Chile. Chile.

Resumen

La destrucción de los tejidos de soporte durante la periodontitis crónica está mediada por proteasas específicas. Producto de la actividad proteolítica se generarán cambios en la composición peptídica y proteica globales del fluido crevicular gingival (FCG). El objetivo de este estudio es evaluar la existencia de patrones de proteicos y peptídicos característicos en el FCG de pacientes con periodontitis crónica y sanos.

Materiales y métodos

Se seleccionaron 8 pacientes con periodontitis crónica moderada a severa y 5 sujetos controles y se obtuvieron muestras de FCG. Las muestras se eluyeron en 2 tampones, y se prepararon en 3 matrices distintas, y se obtuvieron los espectros peptídicos y proteicos mediante espectrometría de masas MALDI-TOF. Se procesaron 3 espectros de cada grupo para la obtención final de espectros diferenciales en enfermos.

Resultados

Se obtuvieron patrones proteicos y peptídicos reproducibles en el FCG. En pacientes con periodontitis crónica encontramos perfiles característicos representados por más de 100 proteínas que no fueron detectables en sujetos sanos.

Conclusiones

Demostramos la utilidad de la aplicación de la espectrometría de masas MALDI-TOF en el análisis de cambios cualitativos en la composición global del FCG y presentamos los perfiles proteicos y peptídicos característicos del FCG en periodontitis crónica y sujetos sanos.

Palabras claves: Periodontitis crónica, FCG, MALDI-TOF, perfiles proteicos.

Abstract

Inflammatory response mediates periodontal tissue destruction occurring in chronic periodontitis. As result, qualitative changes in global peptide and protein GCF composition will generate.

Aim

To evaluate existence of characteristic peptide and protein profiles in gingival crevicular fluid (GCF) from patients with chronic periodontitis and controls.

Methods

Patients with moderate to severe chronic periodontitis (n=8) and healthy controls (n=5) were selected and samples of GCF were obtained. Samples were eluted in 2 different buffers and prepared with 3 matrices to obtain peptide and protein spectra by MALDI-TOF mass spectrometry analysis. Subsequently, 3 spectra from each group were processed to obtain final differential spectra.

Results

Reproducible peptide and protein profiles were obtained from both stages in GCF. In disease samples we found characteristic patterns represented by more than 100 proteins which were undetectable in controls.

Conclusions

We demonstrate for the first time the usefulness of MALDI-TOF mass spectrometry in the analysis of qualitative global changes in GCF composition and we present protein and peptide characteristic GCF profiles associated with chronic periodontitis and healthy periodontium.

Key words: Chronic periodontitis, GCF, MALDI-TOF, protein profiles.

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Trabajo recibido el 31 marzo 2008, Aceptado
Aprobado para su publicación el 13 mayo 2008

Correspondencia autor:
Marcela Hernández Ríos
mhernandezrios@gmail.com

Copyright © 2008.
Sociedad de Periodoncia de Chile, Sociedad de Implantología Oral de Chile y Sociedad de Prótesis y Rehabilitación Oral de Chile.