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Rev Clin Periodoncia Implantol Rehabil Oral. 2009;2:46-49
TRABAJO DE INVESTIGACIÓN [PDF]

Expresión de Formas Solubles de MMP-14 y CXCL12 en Periodontitis Crónica Progresiva

Soluble Forms of MMP-14 and CXCL12 Expression in Progressive ChronicPeriodontitis

Hernández M1,3, Tervahartiala T2, Rivera O3, Dezerega A3, Dutzan N3, Henríquez L3, Sorsa T2

1Profesor Asistente, Departamento de Patología, Facultad de Odontología, Universidad de Chile. Chile
2Departamento de Enfermedades Orales y Maxilofaciales, Hospital Central Helsinki, Instituto de Odontología, Universidad de Helsinki, Helsinki, Finlandia
3Laboratorio de Biología Periodontal, Facultad de Odontología, Universidad de Chile. Chile

Resumen

La perdida de tejidos de soporte durante la periodontitis crónica se asocia con la infiltración de leucocitos inflamatorios y la expresión desregulada de MMPs. CXCL12 es una potente quimioquina, mientras que MMP-14 presenta actividad colagenolitica y además es capaz de activar a otras colagenasas, como las MMPs -8 y -13. En este estudio analizamos la expresión de MMP-14 y CXCL12 en FCG de sujetos con periodontitis crónica progresiva.

Materiales y métodos
Se seleccionaron sujetos con periodontitis crónica progresiva y se tomaron muestras de FCG de sitios activos e inactivos (N=34). Mediante immunowestern blot se caracterizaron las formas solubles de la MMP-14 y CXCL12. La MMP-14 se cuantificó mediante densitometria, mientras que los niveles de CXCL12 se determinaron mediante ELISA.

Resultados
CXCL12 y MMP-14 se identificaron en todos los sujetos con progresión. En sitios activos la MMP-14 soluble demostró una tendencia a aumentar y se encontró una fuerte correlación positiva entre MMP-14 y CXCL12, mientras que en sitios inactivos no se encontró correlación alguna.

Conclusión
MMP-14 aumenta conjuntamente con CXCL12 a nivel de sitios activos y esta asociación podría estar relacionada con la perdida activa de soporte periodontal.

Palabras clave: MMP-14, CXCL12, periodontitis crónica progresiva.

Abstract

Support tissue loss during chronic periodontitis is associated with inflammatory leukocyte infiltrates and upregulation of MMP expression. CXCL12 is a potent leukocyte chemoattractant and MMP-14 is a collagenase capable to activate other collagenases such as MMPs -8 and -13. In this study we analyzed the expression of MMP-14 and CXCL12 in GCF of subjects with progressive chronic periodontitis.

Materials and methods
Chronic periodontitis subjects undergoing disease progression were selected. GCF samples were collected from active and inactive sites (N=34). Soluble MMP-14 and CXCL12 forms were characterized by immunowestern blot. MMP-14 was quantified by densitometric analysis and CXCL12 levels were determined by ELISA.

Results
In active sites, soluble MMP-14 showed a tendency to increase and a strong positive correlation was found between MMP-14 and CXCL12, whereas in inactive sites, no correlation was found.

Conclusions
MMP-14 increases together with CXCL12 in active sites and thus, interactions between these mediators could be associated with active loss of tooth support tissues.

Key words: MMP-14, CXCL12, progressive chronic periodontitis.

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CORRESPONDENCIA AUTOR
Marcela Hernández.
mhernandezrios@gmail.com

Trabajo recibido el 09/06/2009.
Aprobado para su publicación el 05/08/2009.

Copyright © 2009.
Sociedad de Periodoncia de Chile, Sociedad de Implantología Oral de Chile y Sociedad de Prótesis y Rehabilitación Oral de Chile.